Objective To analyzed the hygienic test and benefit analysis of traditional sterilized hand towel and disposable sterile toilet paper before surgical hand disinfection. Methods? From October 2021 to November 2021, the sanitary examination of surgeons and nurses after surgical hand disinfection was selected as the research object, and 100 people were selected according to different professional titles, including 50 doctors and 50 nurses, by self-control method. Control group: in the first week, traditional sterilized hand towels were used for hand-drying, and hygienic tests were performed after surgical hand disinfection. Experimental group: In the second week at the same time, the same people were required to use disposable sterile toilet paper to dry their hands and hygienic tests were performed after surgical hand disinfection. The hygiene and benefits of the two groups were evaluated. Results There was no significant difference in the results of hygienic tests between the two groups. In terms of material cost, the cost of sterilized hand towel was higher than disposable sterile toilet paper. Conclusion? The application of disposable sterile toilet paper before surgical hand disinfection has no influence on the hygienic test of surgical hand disinfection, which can effectively reduce operating costs and save manpower in the operating room.